研究方向：

兽医微生物及其分子生物学/动物疫苗与分子免疫学

已获成果：

研究成果获甘肃省科技进步一等奖（第1）、三等奖（第5）各1项，中国农业科学院成果奖2项、成果转化优秀团队奖1项。获新兽药证书6项，其中一类1项，2类2项，三类3项。授权国家发明专利40余项；以第一或通讯作者发表SCI论文50余篇。

承担项目：

（1）主持“十四五”国家重点研发计划课题：口蹄疫病毒抗原多样性与形成机制研究（2021YFD1800304，2021.05-2025.12），975万元。

（2）主持“十三五”国家重点研发计划课题4子任务：种牛场口蹄疫净化标准（2016YFD0501504，2016.07-2020.12），80万元。

（3）主持国家自然基金面上项目：猪圆环病毒与细小病毒复合空衣壳样粒子的分子设计组装及其免疫原性研（31172336，2012.01-2015.12），58万元。

（4）参加国家自然基金面上项目：口蹄疫病毒广谱非中和抗体识别的抗原结构及其对中和抗体应答的影响（32072873，2021.1-2024.12），60万元。

（5）参加国家自然基金面上项目：猪树突状细胞Clec9A受体鉴定及其介导抗原靶向递呈研究（31372422，2014.07-2017.12），79万元。

文章发表（通讯或第一作者）：

1.Wang T, Da L, Zhao J, Yuan H, Sun Y, Zhang L, Li J, Li K, Zhang J, Sun P, Zhao Z, Zhang Q, Zhang Y, Liu Y, Bai X, Lu Z. Annexin A2 orchestrates CSFV entry route selection via the annexin A2/S100A10 heterotetrameric complex. Vet Microbiol. 2026 Aug;319:111055. doi: 10.1016/j.vetmic.2026.111055.

2.Wang T, Da L, Zhao J, Yuan H, Sun Y, Zhang L, Li K, Zhang J, Sun P, Zhao Z, Zhang Q, Zhang Y, Liu Y, Bai X, Lu Z. ANXA2 stabilizes mTOR at the plasma membrane to facilitate autophagic flux for CSFV release. Vet Microbiol. 2026 Feb;313:110863. doi: 10.1016/j.vetmic.2025.110863. 

3.Huang S, Wu S, Li F, Li P, Sun P, Cao Y, Bao H, Dong K, Yang J, Zhang H, Zhao Q, Sun Y, Li D, Bai X, Fu Y, Yuan H, Ma X, Zhao Z, Zhang J, Wang J, Liu Z, Peng Y, Li K, Hua J, Lu Z, Lei D, Zhang Q. Porcine B cell receptor repertoire uncovers balanced recognition of antigenic structures on serotype Asia1 foot-and-mouth disease virus. PLoS Pathog. 2026 Feb 6;22(2):e1013893. doi: 10.1371/journal.ppat.1013893.

4.Gong X, Yuan H, Wang T, Huang L, Zhao J, Li P, Cai M, Bao H, Sun P, Li K, Ma X, Cao Y, Fu Y, Liu Z, Yang Y, Lu Z, Bai X. ATG16L1 promotes virus trafficking to early endosomes and conducts its degradation by 2BC to benefit foot-and-mouth disease virus replication. Int J Biol Macromol. 2026 Mar;349:150865. doi: 10.1016/j.ijbiomac.2026.150865.

5.Huang S, Yang J, Li F, Zhang H, Cao Y, Zha J, Bao H, Li P, Liang L, Liu Z, Li K, Lu Z, Hua J, Zhang Q. Two residue mutations in VP1 of FMDV serotype A cause significant antigenic variation via reorientation of G-H loop revealed by lineage-specific neutralizing antibodies from the natural hosts. Vet Microbiol. 2025 Dec;311:110748.

6.Sun Y, Huang S, Li F, Huang S, Li P, Zhao Q, Wang T, Bao H, Fu Y, Sun P, Bai X, Yuan H, Ma X, Zhao Z, Zhang J, Wang J, Li D, Zhang Q, Cao Y, Li K, Lu Z, Fan H. Porcine antibodies reveal novel non-neutralizing universal epitopes on FMDV and their overlaps with neutralization sites. Vet Microbiol. 2025 Apr;303:110440. doi: 10.1016/j.vetmic.2025.110440.

7.Cao Y, Li F, Xing X, Zhang H, Zhao Q, Sun P, Fu Y, Li P, Ma X, Zhang J, Zhao Z, Yuan H, Wang J, Wang T, Bao H, Bai X, Li D, Zhang Q, Li K, Lu Z. Preparation and application of porcine broadly neutralizing monoclonal antibodies in an immunoassay for efficiently detecting neutralizing antibodies against foot-and-mouth disease virus serotype O. Microbiol Spectr. 2025 Feb 4;13(2):e0223424.

8.Zhang K, Zha J, Sun P, Ouyang Y, Zhang X, Ma X, Liu F, Li D, Bao H, Cao Y, Bai X, Fu Y, Li K, Yuan H, Zhang J, Zhao Z, Wang J, Zhang Q, Liu Z, Lu Z, Li P. Foot-and-mouth disease vaccine with insertion of a 24-amino acid VP1 G-H loop epitope of the Cathay virus provides broad antigenic coverage. Appl Microbiol Biotechnol. 2025 Sep 27;109(1):206. doi: 10.1007/s00253-025-13545-8.

9.Zha J, Zhang K, Zhang X, Sun P, Li D, Cao Y, Bai X, Ma X, Li K, Yuan H, Fu Y, Zhang J, Zhao Z, Wang J, Bao H, Ou Yang Y, Qi S, Liu Z, Lu Z, Li P. Foot-and-mouth disease virus vaccine with VP1 G-H loop substitution of the Cathay strain broadens antigen spectrum. Appl Microbiol Biotechnol. 2025 Sep 11;109(1):199. doi: 10.1007/s00253-025-13561-8.

10.Sun M, Bao Y, Li K, Zuo Y, Zhang H, Fu Y, Li P, Sun P, Zhao Z, Jiang T, Bai X, Lin M, Lu Z, Cao Y. A chemiluminescence immunoassay for detecting neutralizing antibodies of foot-and-mouth disease virus serotype A. Appl Microbiol Biotechnol. 2025 Oct 18;109(1):232. doi: 10.1007/s00253-025-13616-w.

11.Yuan H, Jiao Y, Gao J, Wang T, Xia Y, Li K, Yang Y, Zhang J, Bao H, Wang L, Sun P, Li D, Li P, Cao Y, Zhao Z, Liu Z, Lu Z, Liu Y, Bai X. Enhancement of immune responses to classical swine fever virus E2 in mice by fusion or mixture with the porcine IL-28B. Appl Microbiol Biotechnol. 2025 Feb 13;109(1):44. doi: 10.1007/s00253-024-13399-6.

12.Zhao Q, Li F, Huang S, Xing X, Sun Y, Li P, Bao H, Fu Y, Sun P, Bai X, Yuan H, Ma X, Zhao Z, Zhang J, Wang J, Wang T, Li D, Zhang Q, Qian P, Cao Y, Li K, Li X, Lu Z. Remapping the spatial distribution of neutralizing sites and their immunodominance on the capsid of different topotypes of FMDV serotype O by site-directed competitive ELISA for detection of neutralizing antibodies. Microbiol Spectr. 2025 Jun 3;13(6): e0334424.

13.Li F, Wu S, Lv L, Huang S, Zhang Z, Zerang Z, Li P, Cao Y, Bao H, Sun P, Bai X, He Y, Fu Y, Yuan H, Ma X, Zhao Z, Zhang J, Wang J, Wang T, Li D, Zhang Q, He J, Liu Z, Lu Z, Lei D, Li K. Discovery, recognized antigenic structures, and evolution of cross-serotype broadly neutralizing antibodies from porcine B-cell repertoires against foot-and-mouth disease virus. PLoS Pathog. 2024 Oct 15;20(10): e1012623. doi: 10.1371/journal.ppat.1012623.

14.Li J, Zhang J, Sun P, Wang J, Li G, Cui Z, Li D, Yuan H, Wang T, Li K, Bai X, Zhao Z, Cao Y, Ma X, Li P, Fu Y, Bao H, Liu Z, Xiao S, Wang X, Lu Z. Porcine reproductive and respiratory syndrome virus nonstructural protein 2 promotes the autophagic degradation of adaptor protein SH3KBP1 to antagonize host innate immune responses by enhancing K63-linked polyubiquitination of RIG-I. PLoS Pathog. 2024 Oct 28;20(10): e1012670. doi: 10.1371/journal.ppat.1012670.

15.Li G, Zhang J, Zhao Z, Wang J, Li J, Xu W, Cui Z, Sun P, Yuan H, Wang T, Li K, Bai X, Ma X, Li P, Fu Y, Cao Y, Bao H, Li D, Liu Z, Zhu N, Tang L, Lu Z. RNF144B negatively regulates antiviral immunity by targeting MDA5 for autophagic degradation. EMBO Rep. 2024 Oct;25(10):4594-4624. doi: 10.1038/s44319-024-00256-w.

16.Cui Z, Liu J, Xie C, Wang T, Sun P, Wang J, Li J, Li G, Qiu J, Zhang Y, Li D, Sun Y, Yin J, Li K, Zhao Z, Yuan H, Bai X, Ma X, Li P, Fu Y, Bao H, Li D, Zhang Q, Liu Z, Cao Y, Zhang J, Lu Z. High-throughput screening unveils nitazoxanide as a potent PRRSV inhibitor by targeting NMRAL1. Nat Commun. 2024 Jun 6;15(1):4813. doi: 10.1038/s41467-024-48807-y.

17.Jiao S, Zhang J, Wang J, Ma X, Li G, Li J, Cui Z, Li D, Li P, Zeng Q, Liu Z, Lu Z, Sun P. Whole-genome analysis of the recombination and evolution of newly identified NADC30-like porcine reproductive and respiratory syndrome virus strains circulated in Gansu province of China in 2023. Front Vet Sci. 2024 Apr 12; 11:1372032. doi: 10.3389/fvets.2024.1372032.

18.Cui Z, Zhang J, Wang J, Liu J, Sun P, Li J, Li G, Sun Y, Ying J, Li K, Zhao Z, Yuan H, Bai X, Ma X, Li P, Fu Y, Bao H, Li D, Zhang Q, Liu Z, Cao Y, Lu Z. Caffeic acid phenethyl ester: an effective antiviral agent against porcine reproductive and Respiratory Syndrome Virus. Antiviral Res. 2024 May; 225:105868. doi: 10.1016/j.antiviral.2024.105868.

19.Li K, He Y, Wang L, Li P, Bao H, Huang S, Zhou S, Zhu G, Song Y, Li Y, Wang S, Zhang Q, Sun P, Bai X, Zhao Z, Lou Z, Cao Y, Lu Z, Liu Z. Conserved antigen structures and antibody-driven variations on foot-and-mouth disease virus serotype A revealed by bovine neutralizing monoclonal antibodies. PLoS Pathog. 2023 Nov 20;19(11):e1011811.

20.Ma X, Huang J, Li K, Ding K, Fu Y, Zhang J, Zhao Z, Li P, Bai X, Li D, Liu X, Zeng Q, Liu Z, Sun P, Lu Z. Development and Evaluation of a Competitive Enzyme-Linked Immunosorbent Assay Based on Swine Monoclonal Antibodies for Detecting Neutralizing Antibodies against Senecavirus A. Microbiol Spectr. 2023: e0459922.

21.Fu Y, Li D, Cao Y, Zhou P, Li K, Zhao Z, Li P, Bai X, Bao H, Wang S, Zhao L, Wang X, Liu Z, Sun P, Lu Z. Development of a double-antibody sandwich ELISA for rapidly quantitative detection of residual non-structural proteins in inactivated foot-and-mouth disease virus vaccines. J Virol Methods. 2023，314:114676.

22.Zhao Z, Huang C, Zhu X, Qi Z, Cao Y, Li P, Bao H, Sun P, Bai X, Fu Y, Li K, Zhang J, Ma X, Wang J, Yuan H, Li D, Liu Z, Zhang Q, Lu Z. Creation of poxvirus expressing foot-and-mouth and peste des petits ruminant disease virus proteins. Appl Microbiol Biotechnol. 2023, 107(2-3):639-650.

23.Zhang J, Li F, Sun P, Wang J, Li K, Zhao Z, Bai X, Cao Y, Bao H, Li D, Zhang J, Liu Z, Lu Z. Downregulation of miR-122 by porcine reproductive and respiratory syndrome virus promotes viral replication by targeting SOCS3. Vet Microbiol. 2022, 275:109595.

24.Zhang J, Gan L, Sun P, Wang J, Li D, Cao Y, Fu Y, Li P, Bai X, Li K, Ma X, Bao H, Chen Y, Zhang J, Liu Z, Lu Z. The long non-coding RNA LNC_000397 negatively regulates PRRSV replication through induction of interferon-stimulated genes. Virol J. 2022, 19(1):40.

25.Li P, Huang S, Zha J, Sun P, Li D, Bao H, Cao Y, Bai X, Fu Y, Ma X, Li K, Yuan H, Zhang J, Zhao Z, Wang J, Zhang K, Chen Y, Zhang Q, Qi S, Liu Z, Lu Z. Evaluation of immunogenicity and cross-reactive responses of vaccines prepared from two chimeric serotype O foot-and-mouth disease viruses in pigs and cattle. Vet Res. 2022 Jul 8;53(1):56.

26.Cao Y, Li K, Xing X, Zhu G, Fu Y, Bao H, Bai X, Sun P, Li P, Zhang J, Ma X, Wang J, Zhao Z, Li D, Liu Z, Lu Z. Development and Validation of a Competitive ELISA Based on Bovine Monoclonal Antibodies for the Detection of Neutralizing Antibodies against Foot-and-Mouth Disease Virus Serotype A. J Clin Microbiol. 2022 Apr 20;60(4):e0214221.

27.He Y, Li K, Wang L, Sun Z, Cao Y, Li P, Sun P, Bao H, Zhou S, Wang S, Bai X, Liu X, Zhao L, Fan X, Liu Z, Lu Z, Yang C, Lou Z. Structures of Foot-and-Mouth Disease Virus with Bovine Neutralizing Antibodies Reveal the Determinant of Intra-serotype Cross-Neutralization. J Virol. 2021 Nov 23;95(24): e0130821.

28.Li K, He Y, Wang L, Li P, Wang S, Sun P, Bao H, Cao Y, Liu X, Zhu G, Song Y, Bai X, Ma X, Fu Y, Yuan H, Zhang J, Wang J, Chen Y, Li D, Lou Z, Liu Z, Lu Z. Two Cross-Protective Antigen Sites on Foot-and-Mouth Disease Virus Serotype O Structurally Revealed by Broadly Neutralizing Antibodies from Cattle. J Virol. 2021 Oct 13;95(21): e0088121.

29.Li K, Zhu G, Zhou S, Sun P, Wang H, Bao H, Fu Y, Li P, Bai X, Ma X, Zhang J, Li D, Chen Y, Liu Z, Cao Y, Lu Z. Isolation and characterization of porcine monoclonal antibodies revealed two distinct serotype-independent epitopes on VP2 of foot-and-mouth disease virus. J Gen Virol. 2021 Jul;102(7). doi: 10.1099/jgv.0.001608.

30.Gao Y, Li P, Ma X, Bai X, Sun P, Du P, Yuan H, Cao Y, Li K, Fu Y, Zhang J, Bao H, Chen Y, Li Z, Lu Z, Liu Z, Li D. The rescue and selection of thermally stable type O vaccine candidate strains of foot-and-mouth disease virus. Arch Virol. 2021 Aug;166(8):2131-2140.

31.Cao Y, Li K, Xing X, Bao H, Huang N, Zhu G, Bai X, Sun P, Fu Y, Li P, Zhang J, Ma X, Li D, Liu Z, Lu Z. Selection of Vaccine Candidate for Foot-and-Mouth Disease Virus Serotype O Using a Blocking Enzyme-Linked Immunosorbent Assay. Vaccines (Basel). 2021 Apr 15;9(4):387.

32.He Y, Li K, Cao Y, Sun Z, Li P, Bao H, Wang S, Zhu G, Bai X, Sun P, Liu X, Yang C, Liu Z, Lu Z, Rao Z, Lou Z. Structures of Foot-and-mouth Disease Virus with neutralizing antibodies derived from recovered natural host reveal a mechanism for cross-serotype neutralization. PLoS Pathog. 2021 Apr 28;17(4):e1009507.

33.Ma XQ, Ling Y, Li PH, Sun P, Cao YM, Bai XW, Li K, Fu YF, Zhang J, Li D, Bao HF, Chen YL, Li ZY, Wang YG, Lu ZJ*, Liu ZX*. Cellular Vimentin Interacts with Foot-and-Mouth Disease Virus Nonstructural Protein 3A and Negatively Modulates Viral Replication. J Virol, 2020, 94(16): e00273-20.

34.Yuan H, Li N, Li PH*, Bai XW, Sun P, Bao HF, Gong XH, Ma XQ, Cao YM, Li K, Fu YF, Zhang J, Li D, Chen YL, Zhang J*, Lu ZJ*, Liu ZX*. Modification of the second translation initiation site restricts the replication of foot-and-mouth disease virus in PK-15 cells. Appl Microbiol Biotechnol, 2020, 104(19): 8455–8466.

35.Yuan H, Li PH, Bao HF, Sun P, Bai XW, Bai QF, Li N, Ma XQ, Cao YM, Fu YF, Li K, Zhang J, Li D, Chen YL, Zhang J, Zeng-Jun Lu, Zaixin Liu*. Engineering viable foot-and-mouth disease viruses with increased acid stability facilitate the development of improved vaccines.Appl Microbiol Biotechnol, 2020, 104(4): 1683–1694.

36.Li PH, Ma XQ, Bai XW, Sun P, Yuan H, Cao YM, Li K, Bao HF, Fu YF, Zhang J, Chen YL, Li D, Li ZY, Lu ZJ*, Liu ZX*. Identification of the largest non-essential regions of the C-terminal portion in 3A protein of foot-and-mouth disease virus for replication in cell culture. Virol J, 2020, 17: 137.

37.Cao Y, Li K, Wang S, Fu Y, Sun P, Li P, Bai X, Zhang J, Ma X, Xing X, Zhou S, Bao H, Li D, Chen Y, Li Z, Lu Z, Liu Z. Implication of broadly neutralizing bovine monoclonal antibodies in the development of an enzyme-linked immunosorbent assay for detecting neutralizing antibodies against foot-and-mouth disease virus serotype O. J Clinical Microbiol, 2019, 57(12): e01030-19.

38.Li K, Wang S, Cao Y, Bao H, Li P, Sun P, Bai X, Fu Y, Ma X, Zhang J, Li D, Chen Y, Liu X, An F, Wu F, Lu Z, Liu Z. Development of foot-and-mouth disease virus-neutralizing monoclonal antibodies derived from plasmablasts of infected cattle and their germline gene usage. Front Immunol, 2019, 10: 2870.

39.Cao Y, Zhou W, Xing X, Zhang J, Fu Y, Li K, Sun P, Li P, Bai X, Ma X, Bao H, Li D, Chen Y, Lu Z, Liu Z. Indirect ELISA using a multi-epitope recombinant protein to detect antibodies against foot-and-mouth disease virus serotype O in pigs. J Virol Methods. 2018, 262:26-31.

40.Bai W, Wang Z, Sun P, Zhang J, Bao H, Cao Y, Chang Y, Liu Z, Li D, Lu Z. The molecular characteristic analysis of PRRSV GSWW/2015 strain and its pathogenicity to pigs. BMC Vet Res. 2018, 14(1):240.

41.Li K, Bao H, Wei G, Cao Y, Li D, Li P, Zhang J, Chen Y, Fu Y, Sun P, Bai X, Ma X, Lu Z*, Liu Z*. Molecular Vaccine Prepared by Fusion of XCL1 to Multi-Epitopes Protein of Foot-and-Mouth Disease Virus enhances Specific Humoral Immune Response in Cattle. Appl Microbiol Biotechnol. 2017, 101(21): 7889-7900.

42.Cao Y, Li D, Fu Y, Bai Q, Chen Y, Bai X, Jing Z, Sun P, Bao H, Li P, Zhang J, Ma X, Lu Z*, Liu Z*. Rational design and efficacy of a multi-epitope recombinant protein vaccine against foot-and-mouth disease virus serotype A in pigs. Antiviral Res. 2017, 140: 133-141.

43.Li K, Wei G, Cao Y, Li D, Li P, Zhang J, Bao H, Chen Y, Fu Y, Sun P, Bai X, Ma X, Lu Z*, Liu Z*. The identification and distribution of cattle XCR1 and XCL1 among peripheral blood cells: new insights into the design of dendritic cells targeted veterinary vaccine. PLoS One. 2017, 12(1): e0170575.

44.Fu Y, Li P, Cao Y, Wang N, Sun P, Shi Q, Ji X, Bao H, Li D, Chen Y, Bai X, Ma X, Zhang J, Lu Z*, Liu Z*. Development of a blocking ELISA Using a monoclonal antibody to a dominant epitope in non-structural protein 3A of foot-and-mouth disease virus, as a matching test for a negative-marker vaccine. PLoS One. 2017, 12(1): e0170560.

45.Zhang J, Sun P, Gan L, Bai W, Wang Z, Li D, Cao Y, Fu Y, Li P, Bai X, Ma X, Bao H, Chen Y, Liu Z, Lu Z. Genome-wide analysis of long noncoding RNA profiling in PRRSV-infected PAM cells by RNA sequencing. Sci Rep. 2017, 7(1):4952.

46.Fu Y, Lu Z, Li P, Cao Y, Sun P, Tian M, Wang N, Bao H, Bai X, Li D, Chen Y, Liu Z. Development of a blocking ELISA based on a monoclonal antibody against a predominant epitope in non-structural protein 3B2 of foot-and-mouth disease virus for differentiating infected from vaccinated animals. PLoS One. 2014, 9(11):e111737.

47.Cao Y, Lu Z, Li D, Fan P, Sun P, Bao H, Fu Y, Li P, Bai X, Chen Y, Xie B, Liu Z. Evaluation of cross-protection against three topotypes of serotype O foot-and-mouth disease virus in pigs vaccinated with multi-epitope protein vaccine incorporated with poly(I:C). Vet Microbiol. 2014, 168(2-4):294-301.

48.Cao Y, Lu Z, Li Y, Sun P, Li D, Li P, Bai X, Fu Y, Bao H, Zhou C, Xie B, Chen Y, Liu Z. Poly(I:C) combined with multi-epitope protein vaccine completely protects against virulent foot-and-mouth disease virus challenge in pigs. Antiviral Res. 2013, 97(2):145-53.

49.Cao Y, Lu Z, Li P, Sun P, Fu Y, Bai X, Bao H, Chen Y, Li D, Liu Z. Improved neutralising antibody response against foot-and-mouth-disease virus in mice inoculated with a multi-epitope peptide vaccine using polyinosinic and poly-cytidylic acid as an adjuvant. J Virol Methods. 2012, 185(1):124-8.

50.Lu Z, Zhang X, Fu Y, Cao Y, Tian M, Sun P, Li D, Liu Z, Xie Q. Expression of the major epitope regions of 2C integrated with the 3AB non-structural protein of foot-and-mouth disease virus and its potential for differentiating infected from vaccinated animals. J Virol Methods. 2010, 170(1-2):128-33.

51.Cao Y, Lu Z, Sun P, Fu Y, Tian F, Hao X, Bao H, Liu X, Liu Z. A pseudotype baculovirus expressing the capsid protein of foot-and-mouth disease virus and a T-cell immunogen shows enhanced immunogenicity in mice. Virol J. 2011, 8:77.

52.Hao X, Lu Z, Kuang W, Sun P, Fu Y, Wu L, Zhao Q, Bao H, Fu Y, Cao Y, Li P, Bai X, Li D, Liu Z. Polymorphic genetic characterization of the ORF7 gene of porcine reproductive and respiratory syndrome virus (PRRSV) in China. Virol J. 2011,8:73.

53.Fu Y, Cao Y, Sun P, Bao H, Bai X, Li P, Li D, Lu Z, Liu Z. Development of a dot immunoblot method for differentiation of animals infected with foot-and-mouth disease virus from vaccinated animals using non-structural proteins expressed prokaryotically. J Virol Methods. 2011, 171(1):234-40.

54.Cao Y, Sun P, Fu Y, Bai X, Tian F, Liu X, Lu Z, Liu Z. Formation of virus-like particles from O-type foot-and-mouth disease virus in insect cells using codon-optimized synthetic genes. Biotechnol Lett. 2010, 32(9):1223-9.

55.Lu Z, Bao H, Cao Y, Sun P, Guo J, Li P, Bai X, Chen Y, Xie B, Li D, Liu Z, Xie Q. Protection of guinea pigs and swine by a recombinant adenovirus expressing O serotype of foot-and-mouth disease virus whole capsid and 3C protease. Vaccine. 2008,26 Suppl 6: G48-53.

56.Lu Z, Cao Y, Guo J, Qi S, Li D, Zhang Q, Ma J, Chang H, Liu Z, Liu X, Xie Q. Development and validation of a 3ABC indirect ELISA for differentiation of foot-and-mouth disease virus infected from vaccinated animals. Vet Microbiol. 2007, 125(1-2):157-69.

主要发明人专利：

1.一种塞内卡病毒猪源单克隆基因工程抗体及其制备方法和应用. 授权日期2024年06月07日, 专利号ZL202010855054.1, 发明人：马雪青;孙普;李坤;李平花;卢曾军;刘在新;付元芳;白兴文,曹轶梅;张婧;祁光宇;李冬;包慧芳.

2.一种基于E2蛋白二聚体的猪瘟病毒管式化学发光抗体检测试剂盒及其应用. 授权日期2024年06月07日, 专利号ZL202211086588.8, 发明人：李坤;包艳芳;林密;何莉;袁红;蒋韬;卢曾军.

3.一种抗塞内卡病毒猪源Fab基因工程抗体及其制备方法和应用. 授权日期2024年08月06日, 专利号ZL202311351414.4, 发明人：马雪青;孙普;卢曾军;李坤;李平花;曹轶梅;付元芳;李冬,张婧;赵志荀;袁红;刘在新.

4.一种抗塞内卡病毒猪源单链基因工程抗体及其制备方法和应用. 授权日期2024年07月30日, 专利号ZL202311351302.9, 发明人：马雪青;孙普;卢曾军;李坤;李平花;曹轶梅;付元芳;李冬,张婧;赵志荀;袁红;刘在新.

5.一种针对当前流行毒株的O型口蹄疫病毒复合表位蛋白疫苗及应用. 授权日期2024年11月29日, 专利号ZL202410078575.9, 发明人：卢曾军;袁红;白兴文;焦云娟;曹轶梅;杨宇轩;李坤;李平花;包慧芳;孙普;李冬;马雪青;赵志荀;付元芳;刘在新.

6.口蹄疫病毒O型和A型广谱中和性猪源单克隆抗体pOA-2及其应用. 授权日期2024年08月30日, 专利号ZL202410667088.6, 发明人：李坤，卢曾军，李凤娟，包慧芳，曹轶梅，李平花，孙普，白兴文，马雪青，付元芳，袁红，赵志荀，张婧，王健，李冬，张强.

7.口蹄疫病毒Asia1型中和性猪源单克隆抗体及其应用. 授权日期2024年08月16日, 专利号ZL202410081359.X, 发明人：李坤，黄书伦，卢曾军，董开恒，张强，包慧芳，曹轶梅，李平花，付元芳，袁红，孙普，白兴文，马雪青，赵志荀，张婧，王健，李冬.

8.口蹄疫病毒O型、A型和Asia1型广谱中和性猪源单克隆抗体及其应用. 授权日期2024年11月15日, 专利号ZL202410667691.4, 发明人：李坤，卢曾军，李凤娟，包慧芳，曹轶梅，李平花，孙普，白兴文，马雪青，付元芳，袁红，赵志荀，张婧，王健，李冬，张强

9.一种O型口蹄疫疫苗候选株及其构建方法和应用，发明专利（ZL202210793288.7），排名第4（2023年6月）

10.一种复制滴度提高的O型口蹄疫病毒株及其构建方法和应用，发明专利（ZL 2022 1 0682771.8），排名第2（2023年9月）

11.一种抗原谱拓展O型口蹄疫病毒株及其构建方法和应用，发明专利（ZL 2021 1 0479816.7），排名第3（2023年3月）

12.一种口蹄疫O型PanAsia-2谱系储备疫苗毒株及其构建方法和应用，发明专利（ZL 2021 1 0589782.7），排名第3（2023年6月）

13.一种双水相萃取浓缩纯化猪塞内卡病毒粒子的方法，发明专利（ZL 2021 1 0196505.X），排名第3（2023年4月）

14.一种用于美洲型猪繁殖与呼吸综合征病毒的荧光RPA检测引物、方法及试剂盒，发明专利（ZL 2022 1 0339139.3），排名第2（2023年6月）

15.一种用于制备PRRSV基因II型表位缺失疫苗毒株的N蛋白表位突变标记及其应用，发明专利（ZL202111638189.3），排名第2（2023年2月）

16.一种猪A型塞内卡病毒中和性抗体、竞争ELISA检测试剂盒及检测方法，发明专利（ZL 2022 1 0205821.3），排名第3（2023年8月）

17.一种口蹄疫病毒致弱突变株及其制备和应用，发明专利（ZL202010877384.0），排名第1（2022年4月）

18.一种用于捕获PRRSV核衣壳蛋白抗体的融合蛋白3AN及其应用，发明专利（ZL202110598098.5），排名第2（2022年01月）

19.PRRSV核衣壳蛋白的猪源单个B细胞抗体及竞争ELISA抗体检测试剂盒，发明专利（ZL202110598030.7），第2（2022年2月）

20.一种口蹄疫病毒非结构蛋白3B优势表位缺失标记毒株及其制备方法和应用，发明专利（ZL202010893827.5），排名第3（2022年4月）

21.FMDV A型牛源中和单抗W145及中和抗体竞争ELISA检测试剂盒，发明专利（ZL202011285447.X），排名第3（2022年9月）

22.一种通用型的口蹄疫病毒结构蛋白抗体及其阻断ELISA检测试剂盒，发明专利（ZL201911022083.3），排名第3（2021年02月）

23.ssc-miR-151-3p在制备调节猪繁殖与呼吸综合征病毒复制的药物中的应用，发明专利（ZL202010729745.7），排名第2（2021年11月）

24.ssc-miR-122在制备调节猪繁殖与呼吸综合征病毒复制的药物中的应用，发明专利（ZL202010729733.4 ），排名第2（2021年8月）

25.一种PRRSV感染相关的lncRNA及其siRNA在抑制病毒复制中的应用，发明专利（ZL201711062600.0），排名第2（2020年03月）

26.A型口蹄疫基因工程复合表位蛋白及疫苗，发明专利（ZL201510897656.2），排名第2（2020年4月）

27.一种猪趋化因子介导的A型口蹄疫靶向性复合表位蛋白及疫苗，发明专利（ZL201610120101.1），排名第2（2020年4月）

28.一种牛趋化因子介导的0型口蹄疫靶向性复合表位蛋白及疫苗，发明专利（ZL201610120102.6），排名第1（2020年4月）

29.一种检测口蹄疫病毒O型保护性抗体的试剂盒和检测方法，发明专利（ZL201910163194.X），排名第3（2020年11月）

30.3B蛋白优势表位缺失的口蹄疫标记疫苗株及其构建方法和应用，发明专利（ZL201710175378.9），排名第3（2020的12月）

31.一种O型口蹄疫病毒突变株及其制备方法和应用，发明专利（ZL201811406871.8），发明专利，排名第3（2020年7月）

32.一种全牛源O型口蹄疫病毒广谱中和抗体的制备方法，发明专利（ZL201810929067.1），排名第2（2019年7月）

33.一种抑制猪繁殖与呼吸综合征病毒的长链非编码RNA，发明专利（ZL201711006075.0），排名第2（2019年10月）

34.一种ELISA试剂盒抗原间接包被酶标板的包被方法，发明专利（ZL 2015 1 0691100.8），排名第1（2017年8月）

35.检测口蹄疫病毒非结构蛋白抗体的单克隆抗体阻断ELISA试剂盒及方法，发明专利（ZL 2012 1 0121363.1），排名第1（2015年4月）

36.一种口蹄疫基因工程混合表位疫苗及其制备方法，发明专利（ZL 2012 1 0480120.7），排名第1（2015年7月）

标准：

1. 国家标准，口蹄疫诊断技术GB/T 18935-2018（第6）

2. 农业行业标准，种畜场口蹄疫免疫无疫控制技术NY/T 4044-2021（第7）

3. 团体标准，猪瘟病毒间接磁微粒 CLIA 抗体检测方法T/CVMA130—2023（第11）

4. 团体标准，口蹄疫病毒非结构蛋白3ABC抗体竞争磁微粒CLIA检测方法T/CVMA129—2023

奖励：

1.卢曾军，李平花，孙普，付元芳，包慧芳，白兴文，王国华，脱浩亮，马雪青，曹轶梅，袁红，李坤，蒋韬，李冬，刘在新．口蹄疫免疫净化技术体系产品创制与产业化.甘肃省科技进步奖，一等奖，2023.7.14.

2.刘在新，卢曾军，李平花，孙普，包慧芳，付元芳，白兴文，曹轶梅，马雪青，李坤，张婧，袁红，李冬，陈应理，赵志荀，张强，李志勇，王健. 中国农业科学院成果转化优秀团队奖，2021年度.

3.谢庆阁，刘在新，曹轶梅，包慧芳，卢曾军，郭建宏，刘湘涛，口蹄疫诊断检疫新技术及试剂盒研制，甘肃省科技进步奖，三等奖，2009；

4.谢庆阁，刘在新，曹轶梅，包慧芳，卢曾军，郭建宏，刘湘涛，口蹄疫诊断检疫新技术及试剂盒研制，中国农业科学院，一等奖，2008.

5.刘在新，李平花，白兴文，孙普，卢曾军，包慧芳，马雪青，曹轶梅，付元芳，袁红，李冬，陈应理，口蹄疫反向遗传学操作技术建立及应用，中国农业科学院杰出科技创新奖，2019.

新兽药证书：

1.卢曾军，付元芳，曹轶梅，孙普，李平花，白兴文，包慧芳，李坤，马雪青，张婧，李冬，陈应理，刘在新，马米玲，林密，黄银君．口蹄疫病毒非结构蛋白3ABC阻断ELSIA抗体检测试剂盒. 2021.7.16， 三类新兽药证书，（2021）新兽药证字46号.

2.刘在新，李平花，包慧芳，卢曾军，孙普，白兴文，殷宏，付元芳，曹轶梅，李冬，陈应理，马雪青，张婧，李坤，张云德. 猪口蹄疫O型病毒3A3B表位缺失灭活疫苗(O/rV-1株)，2017.10.16，一类新兽药证书，（2017）新兽药证字50号.

3.刘在新，孙普，李平花，包慧芳，卢曾军，付元芳，白兴文，曹轶梅，李冬，陈应理，马雪青，张婧，李坤. 口蹄疫O型、A型二价3B蛋白表位缺失灭活疫苗（O/rV-1株-A/rV-2株），2019.8.23，二类新兽药，（2019）新兽药证字52号.

4.刘在新，孙普，卢曾军，蒋韬，付元芳，曹轶梅，包慧芳，李平花，白兴文，李冬，陈应理，吴磊，杨亚民. 口蹄疫病毒非结构蛋白2C3AB抗体检测试纸条，2015.5.27，二类新兽药证书，（2015）新兽药证字23号.

5.刘在新，曹轶梅，卢曾军，郭建宏，谢庆阁，口蹄疫病毒非结构蛋白3ABC间接ELISA抗体检测试剂盒，三类新兽药证书（2006新兽药证字69号），2006.